Publications

Publications

  • Implementing hands-on molecular and synthetic biology education using cell-free technology
  • Huang, A. *, Bryan, B., Kraves, S., Alvarez-Saavedra, E., & Stark, J. C. *
  • Methids Molecular Biology, in press (2021)
  • Synthetic Siglec-9 agonists inhibit neutrophil activation associated with COVID-19
  • Delaveris, C. S., Wilk, A. J., Riley, N. M., Stark, J. C., Yang, S. S., Rogers, A. J., … & Bertozzi, C. R. *
  • ACS Central Science (2021) doi: 10.1021/acscentsci.0c01669
  • A cell-free biosynthesis platform for modular construction of protein glycosylation pathways
  • Kightlinger, W., Duncker, K. E., Ramesh, A., Thames, A. H., Natarajan, A., Stark, J. C., … & Jewett, M. C. *
  • Nature Communications (2019) doi: 10.1038/s41467-019-12024-9
  • Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
  • Jaroentomeechai, T. †, Stark, J. C. †, Natarajan, A., Glasscock, C., Mrksich, M., Jewett, M. C. *, & DeLisa, M. P. *
  • Nature Communications (2018) doi: 10.1038/s41467-018-05110-x
  • A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases
  • Schoborg, J. A. †, Hershewe, J. M. †, Stark, J. C., Kightlinger, W. K., Kath, J. E., Jaroentomeechai, T., … & Jewett, M. C. *
  • Biotechnology & Bioengineering (2017) doi: 10.1002/bit.26502
  • A pipeline for studying and engineering single-subunit oligosaccharyltransferases
  • Jaroentomeechai, T., Zheng, X., Hershewe, J. M., Stark, J. C., Jewett, M. C., & DeLisa, M. P. *
  • Methods in Enzymology (2017) doi: 10.1016/bs.mie.2017.07.011.
  • Cell-free synthetic biology: Engineering beyond the cell
  • in “Synthetic Biology,” J. Craig Venter, Daniel Gibson, Hamilton Smith, Clyde Hutchison, editors
  • Perez, J. G. †, Stark, J. C. †, & Jewett, M. C. *
  • Cold Spring Harbor Perspectives in Biology (2016) doi: 10.1101/cshperspect.a023853
  • Energizing eukaryotic cell-free protein synthesis with glucose metabolism
  • Anderson, M. J. †, Stark, J. C. †, Hodgman, C. E., & Jewett, M. C. *
  • FEBS Letters (2015) doi: 10.1016/j.febslet.2015.05.045
  • Repurposing a bacterial quality control mechanism to enhance production of a fungal endocellulase
  • Boock, J. T., King, B. C., Taw, M., Conrado, R. J., Siu, K.-H., Stark, J. C., … & DeLisa, M. P. *
  • Journal of Molecular Biology (2015) doi: 10.1016/j.jmb.2015.01.003

( † equal contribution; * corresponding author )

Patent

  • Jewett, M. C., Stark, J. C., DeLisa, M. P., & Jaroentomeechai, T. (2020) Method for rapid in vitro synthesis of bioconjugate vaccines via recombinant production of N-glycosylated proteins in prokaryotic cell lysates. US Patent No. 10,829,795.

Patent Applications

  • Bertozzi, C. R., Stark, J. C., Gray, M. A., & Wisnovsky, S. P. (2021) Bispecific molecules and related compositions and methods. US Provisional Patent Application No. 63170297.
  • Jewett, M. C., Stark, J. C., DeLisa, M. P., & Jaroentomeechai, T. (published 2018) Cell-free glycoprotein synthesis (CFGpS) in prokaryotic cell lysates enriched with components for glycosylation. US Patent Application Serial No. 62/273,124.
  • Jewett, M. C., Anderson, M. J., Stark, J. C., & Hodgman, C. E. (published 2017) Methods for activating natural energy metabolism for improved yeast cell-free protein synthesis. US Patent Application Serial No. 15/639,364.

Invited Talks

  • "Engineering (Glyco)immunology." Chemical Engineering and Materials Science Joint Bioengineering Group Meeting, University of Minnesota, Minneapolis, MN, 13 Oct 2021.
  • "Understanding and engineering the immune response to glycoconjugates." Cottrell Emerging Scholar Seminar, University of Oregon, Eugene, OR, 29 Jan 2021.
  • "Cell-free technologies for on-demand glycoprotein biomanufacturing and hands-on biology education." Curie Symposium, University of Washington Institute for Protein Design, Seattle, WA, 21 Nov 2019.
  • "Using sugars to understand and program the immune system." Hanna H. Gray Fellows Finalist Symposium, Howard Hughes Medical Institute, Chevy Chase, MD, 25 Jun 2019.

Presentations

  • "Interrogating Siglec immune checkpoints in cancer using interaction proteomics." American Institute of Chemical Engineers Annual Meeting, virtual, 19 Nov 2020 (Oral presentation).
  • "Cell-free technologies for customizable glycoprotein therapeutic and vaccine production." Mammalian Synthetic Biology Workshop, Evanston, IL, 18 May 2019 (Poster).
  • "Cell-free technologies for customizable glycoprotein therapeutic and vaccine production." Glycobiology Gordon Research Conference, Lucca (Barga), IT, 9-14 Mar 2019 (Poster).
    Poster Presentation Award.
  • "Cell-free technologies for customizable glycoprotein synthesis and point-of-care conjugate vaccine biomanufacturing." Glycobiology Gordon Research Seminar, Lucca (Barga), IT, 9 Mar 2019 (Oral presentation).
  • "Cell-free technologies for on-demand glycoprotein biomanufacturing." International Conference on Biomolecular Engineering, Newport Beach, CA, 9 Jan 2019 (Oral presentation).
  • "Cell-free glycoprotein synthesis enables portable, on-demand conjugate vaccine production." Central US Synthetic Biology Workshop, Evanston, IL, 7 Sept 2018 (Oral presentation).
  • "Customizable, in vitro protein glycosylation for antibacterial and anti-cancer vaccines." American Association for Cancer Research Annual Meeting, Chicago, IL, 17 Apr 2018 (Poster).
  • "BioBits Bright: A fluorescent synthetic biology education kit." Engineering Biology Research Consortium Retreat, Seattle, WA, 24 March 2018 (Oral presentation and poster).
  • "Care packages: Developing a cell-free glycoprotein synthesis platform for portable, on-demand antibacterial vaccine production." American Chemical Society National Meeting, New Orleans, LA, 20 Mar 2018 (Oral presentation).
    Best Presentation Award.
  • "Portable educational tools for synthetic biology." American Chemical Society National Meeting, New Orleans, LA, 20 Mar 2018 (Poster).
  • "Mix and match sweets: A cell-free glycoprotein synthesis system for portable, on-demand antibacterial vaccine production." Engineering Biology Research Consortium Retreat, Evanston, IL, 25 Mar 2017 (Oral presentation).
  • "Mix and match sweets: A cell-free glycoprotein synthesis system for portable, on-demand vaccine production." American Institute of Chemical Engineers Annual Meeting, San Francisco, CA, 14 Nov 2016 (Oral presentation).
    AIChE Division 15 Oral Presentation Award.
  • "Mix and match sweets: Modular, coordinated cell-free transcription, translation, and glycosylation of proteins using selectively enriched Escherichia coli cell lysates." Synthetic Biology: Engineering, Evolution, and Design Conference, Chicago, IL, 19 Jul 2016 (Oral presentation).
  • "Energizing yeast cell-free protein synthesis with glucose metabolism." International Conference on Biomolecular Engineering, Austin, TX, 11-14 Jan 2015 (Poster).
    2nd Place Poster Award.
  • "Energizing yeast cell-free protein synthesis with glucose metabolism." NIH Biotechnology Training Program Biotechnology Day, Northwestern University, Evanston, IL, 06 Oct 2014 (Poster).
  • "High concentration mAb aggregation, thermal unfolding, and the curious case of mAb A." Pharmaceutical Development Research Forum, Genentech, Inc., South San Francisco, CA, 23 Apr 2013 (Poster).