Publications

Publications

  • ( † equal contribution; * corresponding author )
  • Antibody-lectin chimeras for glyco-immune checkpoint blockade
  • Stark, J. C. †, Gray, M. A. †, Wisnovsky, S. P. , Ibarlucea-Benitez, I., Riley, N. M., Ribi, M. K., Lustig, M., Errington, W. J., Bruncsics, B., Sarkar, C. A., Valerius, T., Ravetch, J. V., & Bertozzi, C. R. †
  • bioRxiv (2022) doi: 10.1101/2022.10.26.513931
  • Rapid biosynthesis of glycoprotein therapeutics and vaccines from freeze-dried bacterial cell lysates
  • Stark, J. C. *, Jaroentomeechai, T., Warfel, K. F., Hershewe, J. M., DeLisa, M. P. *, & Jewett, M. C. *
  • Nature Protocols, in press
  • Genome-wide, bidirectional CRISPR screens identify mucins as critical host factors modulating SARS-CoV-2 infection
  • Biering, S. B. †, Sarnik, S. A. †, … , Stark, J. C., … Harris, E. *, Konermann, S. *, & Hsu, P. D. *
  • Nature Genetics (2022) doi: 10.1038/s41588-022-01131-x
  • Targeting hypersialylation in multiple myeloma represents a novel therapeutic strategy to enhance NK cell-mediated tumor responses
  • Daly, J., Sarkar, S., Natoni, A., Stark, J. C., Riley N. M., Bertozzi, C. R., Carlsten, M. *, & O’Dwyer, M. *
  • Blood Advances (2022) doi: 10.1182/bloodadvances.2021006805
  • Implementing hands-on molecular and synthetic biology education using cell-free technology
  • Huang, A. *, Bryan, B., Kraves, S., Alvarez-Saavedra, E., & Stark, J. C. *
  • Methods Molecular Biology (2022) doi: 10.1007/978-1-1998-8_25
  • Synthetic Siglec-9 agonists inhibit neutrophil activation associated with COVID-19
  • Delaveris, C. S., Wilk, A. J., Riley, N. M., Stark, J. C., Yang, S. S., Rogers, A. J., … & Bertozzi, C. R. *
  • ACS Central Science (2021) doi: 10.1021/acscentsci.0c01669
  • A cell-free biosynthesis platform for modular construction of protein glycosylation pathways
  • Kightlinger, W., Duncker, K. E., Ramesh, A., Thames, A. H., Natarajan, A., Stark, J. C., … & Jewett, M. C. *
  • Nature Communications (2019) doi: 10.1038/s41467-019-12024-9
  • Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
  • Jaroentomeechai, T. †, Stark, J. C. †, Natarajan, A., Glasscock, C., Mrksich, M., Jewett, M. C. *, & DeLisa, M. P. *
  • Nature Communications (2018) doi: 10.1038/s41467-018-05110-x
  • A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases
  • Schoborg, J. A. †, Hershewe, J. M. †, Stark, J. C., Kightlinger, W. K., Kath, J. E., Jaroentomeechai, T., … & Jewett, M. C. *
  • Biotechnology & Bioengineering (2017) doi: 10.1002/bit.26502
  • A pipeline for studying and engineering single-subunit oligosaccharyltransferases
  • Jaroentomeechai, T., Zheng, X., Hershewe, J. M., Stark, J. C., Jewett, M. C., & DeLisa, M. P. *
  • Methods in Enzymology (2017) doi: 10.1016/bs.mie.2017.07.011.
  • Cell-free synthetic biology: Engineering beyond the cell
  • in “Synthetic Biology,” J. Craig Venter, Daniel Gibson, Hamilton Smith, Clyde Hutchison, editors
  • Perez, J. G. †, Stark, J. C. †, & Jewett, M. C. *
  • Cold Spring Harbor Perspectives in Biology (2016) doi: 10.1101/cshperspect.a023853
  • Energizing eukaryotic cell-free protein synthesis with glucose metabolism
  • Anderson, M. J. †, Stark, J. C. †, Hodgman, C. E., & Jewett, M. C. *
  • FEBS Letters (2015) doi: 10.1016/j.febslet.2015.05.045
  • Repurposing a bacterial quality control mechanism to enhance production of a fungal endocellulase
  • Boock, J. T., King, B. C., Taw, M., Conrado, R. J., Siu, K.-H., Stark, J. C., … & DeLisa, M. P. *
  • Journal of Molecular Biology (2015) doi: 10.1016/j.jmb.2015.01.003

Patent

  • Jewett, M. C., Stark, J. C., DeLisa, M. P., & Jaroentomeechai, T. (2020) Method for rapid in vitro synthesis of bioconjugate vaccines via recombinant production of N-glycosylated proteins in prokaryotic cell lysates. US Patent No. 10,829,795.

Patent Applications

  • Bertozzi, C. R., Stark, J. C., Gray, M. A., & Wisnovsky, S. P. (2021) Bispecific molecules and related compositions and methods. PCT/US Patent Application No. 22/23166.
  • Jewett, M. C., Stark, J. C., DeLisa, M. P., & Jaroentomeechai, T. (published 2018) Cell-free glycoprotein synthesis (CFGpS) in prokaryotic cell lysates enriched with components for glycosylation. US Patent Application Serial No. 62/273,124.
  • Jewett, M. C., Anderson, M. J., Stark, J. C., & Hodgman, C. E. (published 2017) Methods for activating natural energy metabolism for improved yeast cell-free protein synthesis. US Patent Application Serial No. 15/639,364.

Invited Talks

  • "Engineering glycoimmunology." Rising Stars in Engineering Health Workshop, Johns Hopkins University, 18 Nov 2022.
  • "Targeting sugars for immunotherapy with antibody-lectin chimeras." Broad Institute of MIT and Harvard, Cambridge, MA, 13 Oct 2022.
  • "Engineering glycoimmunology." American Chemical Society Best of BIOT Webinar, virtual, 11 Aug 2022.
  • "Engineering glycoimmunology." University of Washington, Seattle, WA, 8 Aug 2022.
  • "Engineering glycoimmunology." Sloan Kettering Institute/Memorial Sloan Kettering Cancer Center, New York, NY, 15 July 2022.
  • "Engineering glycoimmunology." Leading Edge Symposium, Immunology, virtual, 20 July 2022.
  • "Engineering glycoimmunology." University of California, Santa Barbara, Santa Barbara, CA, 10 Mar 2022.
  • "Engineering glycoimmunology." University of Minnesota, Minneapolis, MN, 13 Oct 2021.
  • "Understanding and engineering the immune response to glycoconjugates." University of Oregon, Eugene, OR, 29 Jan 2021.
  • "Cell-free technologies for on-demand glycoprotein biomanufacturing and hands-on biology education." Institute for Protein Design/University of Washington, Seattle, WA, 21 Nov 2019.
  • "Using sugars to understand and program the immune system." Howard Hughes Medical Institute, Chevy Chase, MD, 25 Jun 2019.